abstract：:Large numbers (10(4) to greater than 10(5)/ml) of Type I water-resistant Giardia lamblia cysts were produced in vitro under conditions that are characteristic of the human intestinal lumen. We define Type I cyst morphology as oval shaped, smooth, and refractile, with cyst wall, axostyle, and median body visible in rel...

journal_title：Experimental parasitology

authors： Gillin FD,Boucher SE,Rossi SS,Reiner DS

更新日期：1989-08-01 00:00:00

abstract：:Chromosome-sized DNA molecules from Trypanosoma cruzi clone Dm 28c were analyzed and compared with other T. cruzi strains and monogenetic trypanosomatids by orthogonal field alteration gel electrophoresis. The results showed that T. cruzi Dm 28c displays at least 18 chromosomes ranging from 550 to more than 1500 kb an...

journal_title：Experimental parasitology

authors： Aymerich S,Goldenberg S

更新日期：1989-08-01 00:00:00

abstract：:Antimony unresponsiveness in mucocutaneous and visceral leishmaniasis is a serious clinical problem. Information on the mechanisms and characteristics of drug resistance in parasites that suggest chemotherapeutic strategies to overcome resistance is of practical importance. We developed nine lines of Leishmania resist...

journal_title：Experimental parasitology

authors： Grögl M,Oduola AM,Cordero LD,Kyle DE

更新日期：1989-07-01 00:00:00

abstract：:In 1912 Blastocystis hominis was identified as a new species and classified as a yeast (Brumpt 1912). In the early 1920s several groups confirmed its classification as a yeast, specifically a member of the genus Schizosaccharomyces (discussed by Zierdt et al. 1967). Apart from an occasional case report, the classifica...

journal_title：Experimental parasitology

authors： Johnson AM,Thanou A,Boreham PF,Baverstock PR

更新日期：1989-04-01 00:00:00

abstract：:In Trichostrongylus colubriformis-infected rabbits, epithelial cell migration rates and cell transit times along the villi were compared by radioautography on histological slides to normal values from noninfected small intestine. Regions of gut with high (upper jejunum) and low (ileum) burdens of worms were both exami...

journal_title：Experimental parasitology

authors： Hoste H,Mariana JC

更新日期：1989-04-01 00:00:00

abstract：:Giardia lamblia undergo surface antigenic variation in vitro and in vivo. The presence of variant trophozoites can be detected in clones after exposure to cytotoxic monoclonal antibodies. Surviving Giardia (progeny) no longer possess the initial major surface antigen which is replaced by new antigens. Exposure of a cl...

journal_title：Experimental parasitology

authors： Nash TE

更新日期：1989-02-01 00:00:00

abstract：:Five adjuvants were examined for their ability to potentiate the immune response of mice to soluble antigens from adult Nematospiroides dubius prepared by affinity chromatography against antibodies from repeatedly infected mice as ligands (IMIgAg). Immunized mice were better protected against N. dubius by IMIgAg injec...

journal_title：Experimental parasitology

authors： Monroy FG,Adams JH,Dobson C,East IJ

更新日期：1989-01-01 00:00:00

abstract：:A genetically homogeneous population of Plasmodium falciparum prepared by a single erythrocyte micromanipulation technique was used to produce lines of P. falciparum resistant to mefloquine hydrochloride in vitro. Parasites were maintained in a culture medium containing gradually increased concentrations of mefloquine...

journal_title：Experimental parasitology

authors： Oduola AM,Milhous WK,Weatherly NF,Bowdre JH,Desjardins RE

更新日期：1988-12-01 00:00:00

abstract：:The identification of nucleolus organizer regions (NORs) and the qualitative description of silver nitrate staining patterns in spermatogenesis of Paragonimus ohirai are herein reported. The NORs of P. ohirai are located on the short arm of chromosome 4 and are associated with a large amount of constitutive heterochro...

journal_title：Experimental parasitology

authors： Hirai H

更新日期：1988-12-01 00:00:00

abstract：:Inbred strains of mice differ in their susceptibility and resistance to challenge infections with Nematospiroides dubius. In our studies, F1 hybrid mice from resistant SJL and susceptible CBA parents were resistant to N. dubius challenge infections. Only 22% of backcrosses to SJL were susceptible while backcrosses to ...

journal_title：Experimental parasitology

authors： Enriquez FJ,Zidian JL,Cypess RH

更新日期：1988-10-01 00:00:00

abstract：:Trypanosoma musculi is a hemoflagellate parasite producing a characteristic course of infection in the mouse, terminating in elimination of the parasite from the vascular system with permanent immunity to reinfection. While this elimination is known to require functional T lymphocytes, the role of these cells has rema...

journal_title：Experimental parasitology

authors： House RV,Dean JH

更新日期：1988-10-01 00:00:00

abstract：:An NADP-linked glutamate dehydrogenase (EC 1.4.1.4) was found in the soluble fraction of Trichomonas vaginalis. Its molecular weight was about 230,000 (gel filtration). The enzyme, partially purified by diafiltration and hydroxyapatite column chromatography, was heat stable (1 hr at 57 C). It catalyzed both the aminat...

journal_title：Experimental parasitology

authors： Turner AC,Lushbaugh WB

更新日期：1988-10-01 00:00:00

abstract：:The chemical properties of the primary antileishmanial agent sodium stibogluconate (Pentostam), and the interaction of Pentostam with Leishmania mexicana amastigotes, have been investigated with the aid of [125Sb]Pentostam. The molecular weight by P2 chromatography showed [125Sb]Pentostam to be of multiple species of ...

journal_title：Experimental parasitology

authors： Berman JD,Grogl M

更新日期：1988-10-01 00:00:00

abstract：:Factors which control the expression of the refractory or susceptible condition to infection with Dirofilaria immitis in the mosquito. Aedes aegypti, were investigated using three protocols. (1) Microfilariae and prelarvae were injected into the hemocoel of susceptible A. aegypti. Some microfilariae and prelarvae deve...

journal_title：Experimental parasitology

authors： Nayar JK,Knight JW,Bradley TJ

更新日期：1988-06-01 00:00:00

abstract：:There is no evidence that Hymenolepis diminuta can carry out sulphoconjugation reactions. Neither whole worms nor worm extracts were able to sulphate 4-methylumbelliferone. No sulphotransferase activity could be demonstrated in H. diminuta using a variety of substrates, nor was H. diminuta capable of synthesising the ...

journal_title：Experimental parasitology

authors： Raines PS,Barrett J

更新日期：1988-04-01 00:00:00

abstract：:Homogenates of Giardia lamblia trophozoites exhibited the following hydrolase activities: acid phosphatase (EC 3.1.3.2), proteinase (EC 3.1.4) with urea-denatured hemoglobin and N-benzoyl-DL-arginine-2-naphthylamide as substrates, deoxyribonuclease (EC 3.1.4.5), and ribonuclease (EC 2.7.7.16). beta-N-Acetylglucosamini...

journal_title：Experimental parasitology

authors： Lindmark DG

更新日期：1988-02-01 00:00:00

abstract：:Calfluxin (CaFl), one of the gonadotropic hormones of Lymnaea stagnalis, stimulates the influx of Ca2+ into the mitochondria of the cells of the albumen gland, one of the accessory sex organs of the snail. This effect is suppressed in glands of noninfected snails by an agent (schistosomin) present in the hemolymph of ...

journal_title：Experimental parasitology

authors： de Jong-Brink M,Elsaadany MM,Boer HH

更新日期：1988-02-01 00:00:00

abstract：:Freeze-fracturing has been used to study the architecture of the pellicular complex of the gametocytes of Plasmodium falciparum. The gametocyte is surrounded by three membranes and a layer of subpellicular microtubules. During freeze-fracturing, each of the three membranes is split along its hydrophobic interior to yi...

journal_title：Experimental parasitology

authors： Meszoely CA,Erbe EF,Steere RL,Trosper J,Beaudoin RL

更新日期：1987-12-01 00:00:00

abstract：:The rate of phosphorylation of 2-deoxyglucose (2DG) was determined by sequential pulsing of schistosomes (Schistosoma mansoni, S. japonicum, and S. haematobium) with 3H- and 14C-labeled 2-deoxy-D-glucose. Subsequent column chromatographic separation of the neutral [3H]2DG and [14C]2DG from the 3H- and 14C-labeled 2-de...

journal_title：Experimental parasitology

authors： Cornford EM,Fitzpatrick AM

更新日期：1987-12-01 00:00:00

abstract：:Infective larvae of Strongyloides spp. have been shown to contain azocollytic enzymes which may aid in host skin penetration. Attempts to demonstrate classical, neutral pH-active collagenase activity in Strongyloides ratti were unsuccessful. In the current study, we investigated the presence of acidic collagenolytic a...

journal_title：Experimental parasitology

authors： Rege AA,Dresden MH

更新日期：1987-12-01 00:00:00

abstract：:By using a sensitive fluorometric method with Val-Leu-Gly-Arg-3-amino-9-ethylcarbazole (VLGR-AEC) as a substrate, two endopeptidase activities were identified in two fractions of Sephacryl S-200 gel filtration from soluble P. berghei and P. chabaudi extracts. Controls with normal mouse erythrocytes, with leukocytes, a...

journal_title：Experimental parasitology

authors： Bernard F,Mayer R,Picard I,Deguercy A,Monsigny M,Schrevel J

更新日期：1987-08-01 00:00:00

abstract：:There are alterations in the proteins synthesized during different stages of development of Schistosoma mansoni. The protein profiles at different stages were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. When stained by Coomassie blue, no significant differences were seen in protein profiles ...

journal_title：Experimental parasitology

authors： Yuckenberg PD,Poupin F,Mansour TE

更新日期：1987-06-01 00:00:00

abstract：:Three clones of Entamoeba histolytica (L-6, C93, C919) were isolated by mutagenesis with ethyl methanesulfonate from the axenic strain HM1:IMSS and were studied for adherence, cytolytic, and soluble galactose inhibitable lectin activity. Avirulent clones adhered to and killed fewer Chinese hamster ovary cells than HM1...

journal_title：Experimental parasitology

authors： Orozco E,Rodriquez MA,Murphy CF,Salata RA,Petri WA,Smith RD,Ravdin JI

更新日期：1987-04-01 00:00:00

abstract：:Whole cell extracts of 10 clones of bloodstream forms of African trypanosomes representing two strains of Trypanosoma brucei gambiense, one strain of T. b. rhodesiense and one strain of T. b. brucei were fractionated on sodium dodecyl sulfate-polyacrylamide gels, electrophoretically transferred to nitrocellulose paper...

journal_title：Experimental parasitology

authors： Frommel TO,Balber AE

更新日期：1987-02-01 00:00:00

abstract：:A mode of action of the inducible treatment with trypsin for the development of Mesocestoides lineatus tetrathyridium to adult was analyzed by administering various agents effective on Ca2+-dependent metabolic pathways in the cells: protein kinase C activators such as a synthetic diacylglycerol, 1-oleoyl-2-acetylglyce...

journal_title：Experimental parasitology

authors： Kawamoto F,Fujioka H,Kumada N,Kojima K

更新日期：1986-12-01 00:00:00

abstract：:Extramedullar proliferation of eosinophil granulocytes can be induced in mice chronically infected with Schistosoma mansoni, by intraperitoneal implants of glass coverslips. Immature eosinophils are located in discrete foci on glass implants; they are not correlated with the eosinophil population of the peritoneal cav...

journal_title：Experimental parasitology

authors： Borojevic R,Cury El-Cheikh MC,Nicola MH

更新日期：1986-12-01 00:00:00

abstract：:Phlebotomus papatasi is susceptible to Leishmania major which it transmits in nature, but is resistant to L. donovani. The present study compares the effect of L. major and L. donovani on the proteolytic activity of P. papatasi gut enzymes. The experiments measured digestion of C14-labeled globin by gut homogenates of...

journal_title：Experimental parasitology

authors： Schlein Y,Romano H

更新日期：1986-12-01 00:00:00

abstract：:The human complement component C3b is known to bind in vitro to the surfaces of all developmental stages of schistosomes as a consequence of complement activation by the alternative pathway. C3b bound to Schistosoma mansoni parasites has now been used in combination with fluorescent labeled antibodies against C3b to l...

journal_title：Experimental parasitology

authors： Ruppel A,McLaren DJ

更新日期：1986-10-01 00:00:00

abstract：:This is the first report establishing the existence of glycolipids synthesized by plasmodia, in particular Plasmodium falciparum. Trophozoites, schizonts, gametocytes, and gametes were metabolically labeled in vitro with [3H]glucosamine, [3H]galactose, [3H]glucose, [3H]mannose, [3H]fucose, [32P]inorganic phosphate, or...

journal_title：Experimental parasitology

authors： Sherwood JA,Spitalnik SL,Aley SB,Quakyi IA,Howard RJ

更新日期：1986-08-01 00:00:00

abstract：:One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion. The proteins whic...

journal_title：Experimental parasitology

authors： Le Febvre RB,Hill GC

更新日期：1986-08-01 00:00:00

abstract：:Crude salivary gland derived proteins from Amblyomma americanum ticks were analyzed by physiochemical (gel filtration and ion exchange chromatography) and immunochemical guinea pig IgG1 (anti-tick immunoaffinity column) techniques for the presence of antigens responsible for the induction of host immune resistance res...

journal_title：Experimental parasitology

authors： Brown SJ,Askenase PW

更新日期：1986-08-01 00:00:00

abstract：:Two species of glycoproteins from Leishmania braziliensis promastigotes of apparent molecular weights of 53,000 (glycoprotein 53) and 47,000 (glycoprotein 47) were localized. Four lectins with different sugar specificities bound to the blotting sheet to which the electrophoretically separated materials were transferre...

journal_title：Experimental parasitology

authors： Nagakura K,Tachibana H,Kaneda Y,Nakae T

更新日期：1986-06-01 00:00:00

abstract：:Mature males and females of Schistosoma mansoni were incubated for 24 hr in medium containing [3H]cholesterol. Worms thus labeled were paired for 24 hr with unlabeled partners, in vitro or in vivo by surgical implantation into hamsters. Controls consisted of additional unlabeled worms that did not pair. Scintillation ...

journal_title：Experimental parasitology

authors： Popiel I,Basch PF

更新日期：1986-06-01 00:00:00

abstract：:Both the Weybridge strain and a mixture of six laboratory and field strains of Eimeria maxima have been attenuated by selection for early maturation of oocysts during serial passage in chickens. The prepatent times of the resultant precocious lines produces after selection were reduced from approximately 120 hr to les...

journal_title：Experimental parasitology

authors： McDonald V,Shirley MW,Bellatti MA

更新日期：1986-04-01 00:00:00

abstract：:Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecifi...

journal_title：Experimental parasitology

authors： Tomisato M,Nariuchi H,Ueno J,Nogami S,Tanaka H,Aoki Y,Nakajima Y

更新日期：1986-02-01 00:00:00

abstract：:A new type of cell has been identified in cercariae of Schistosoma mansoni. The perikarya (cell bodies) of these cells were located in the body (midsegment), in an area oral to the acetabulum (ventral sucker). Cytoplasmic processes extending from the perikarya ramified throughout the parenchyma of the anterior organ (...

journal_title：Experimental parasitology

authors： Dorsey CH,Cousin CE

更新日期：1986-02-01 00:00:00

abstract：:We show that a purified preparation of the prominent tartrate-resistant acid phosphatase (E.C.3.1.3.2), isolated from the external surface of the intracellular parasite Leishmania donovani (promastigote form), inhibits toxic oxidative metabolite production of neutrophils. Preincubation of a neutrophil suspension (2.5 ...

journal_title：Experimental parasitology

authors： Remaley AT,Glew RH,Kuhns DB,Basford RE,Waggoner AS,Ernst LA,Pope M

更新日期：1985-12-01 00:00:00

abstract：:In mice concurrently infected with Hymenolepis citelli and Nematospiroides dubius, survival of the tapeworm was prolonged, and there was an impairment of the efferent arm of the response to the cestode. The immunological rejection of a six cysticercoid primary H. citelli infection was delayed by the N. dubius infectio...

journal_title：Experimental parasitology

authors： Alghali ST,Hagan P,Robinson M

更新日期：1985-12-01 00:00:00

abstract：:Three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. Ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female Ascaris suum. Perienteric fluid and the body wall did not contain measur...

journal_title：Experimental parasitology

authors： Fleming MW

更新日期：1985-10-01 00:00:00

abstract：:A monoclonal antibody specific for the Theileria parva sporozoite, which recognizes a determinant on the surface coat and blocks sporozoite infectivity, was used to investigate the presence of the determinant on other stages of the parasite lifecycle. Immunofluorescence techniques did not demonstrate this determinant ...

journal_title：Experimental parasitology

authors： Dobbelaere DA,Webster P,Leitch BL,Voigt WP,Irvin AD

更新日期：1985-08-01 00:00:00